Pitfall or pratfall? Behavioral differences in infant learning from falling.

Researchers routinely infer learning and other unobservable psychological functions based on observable behavior. But what behavioral changes constitute evidence of learning? The standard approach is to infer learning based on a single behavior across individuals, including assumptions about the direction and magnitude of change (e.g., everyone should avoid falling repeatedly on a treacherous obstacle). Here we illustrate the benefits of an alternative "multiexpression, relativist, agnostic, individualized" approach. We assessed infant learning from falling based on multiple behaviors relative to each individual's baseline, agnostic about the direction and magnitude of behavioral change. We tested infants longitudinally (10.5-15 months of age) over the transition from crawling to walking. At each session, infants were repeatedly encouraged to crawl or walk over a fall-inducing foam pit interspersed with no-fall baseline trials on a rigid platform. Our approach revealed two learning profiles. Like adults in previous work, "pit-avoid" infants consistently avoided falling. In contrast, "pit-go" infants fell repeatedly across trials and sessions. However, individualized comparisons to baseline across multiple locomotor, exploratory, and social-emotional behaviors showed that pit-go infants also learned at every session. But they treated falling as an unimpactful "pratfall" rather than an aversive "pitfall." Pit-avoid infants displayed enhanced learning across sessions and partial transfer of learning from crawling to walking, whereas pit-go infants displayed neither. Thus, reliance on a predetermined, "one-size-fits-all" behavioral expression of a psychological function can obscure different behavioral profiles and lead to erroneous inferences. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Response of the soil microbial community to petroleum hydrocarbon stress shows a threshold effect: research on aged realistic contaminated fields.

Introduction: Microbes play key roles in maintaining soil ecological functions. Petroleum hydrocarbon contamination is expected to affect microbial ecological characteristics and the ecological services they provide. In this study, the multifunctionalities of contaminated and uncontaminated soils in an aged petroleum hydrocarbon-contaminated field and their correlation with soil microbial characteristics were analyzed to explore the effect of petroleum hydrocarbons on soil microbes. Methods: Soil physicochemical parameters were determined to calculate soil multifunctionalities. In addition, 16S high-throughput sequencing technology and bioinformation analysis were used to explore microbial characteristics. Results: The results indicated that high concentrations of petroleum hydrocarbons (565-3,613 mg•kg-1, high contamination) reduced soil multifunctionality, while low concentrations of petroleum hydrocarbons (13-408 mg•kg-1, light contamination) might increase soil multifunctionality. In addition, light petroleum hydrocarbon contamination increased the richness and evenness of microbial community (p < 0.01), enhanced the microbial interactions and widened the niche breadth of keystone genus, while high petroleum hydrocarbon contamination reduced the richness of the microbial community (p < 0.05), simplified the microbial co-occurrence network, and increased the niche overlap of keystone genus. Conclusion: Our study demonstrates that light petroleum hydrocarbon contamination has a certain improvement effect on soil multifunctionalities and microbial characteristics. While high contamination shows an inhibitory effect on soil multifunctionalities and microbial characteristics, which has significance for the protection and management of petroleum hydrocarbon-contaminated soil.

Response of soil microbial communities to petroleum hydrocarbons at a multi-contaminated industrial site in Lanzhou, China.

Total petroleum hydrocarbon (TPH) contamination poses threats to ecological systems and human health. Many studies have reported its negative impacts on soil microbes, but limited information is known about microbial change and response to multiple TPH contamination events. In this study, we investigated TPH contamination level, microbial community structure and functional genes at a multi-contaminated industrial site in Lanzhou, where a benzene spill accident caused the drinking water crisis in 2014. TPHs distribution in soils and groundwater indicated multiple TPH contamination events in history, and identified the spill location where high TPH level (6549 mg kg-1) and high ratio of low-molecular-weight TPHs (>80%) were observed. In contrast, TPH level was moderate (349 mg kg-1) and the proportion of low-molecular-weight TPHs was 44% in soils with a long TPH contamination history. After the spill accident, soil bacterial communities became significant diverse (p = 0.047), but the dominant microbes remained the same as Pseudomonadaceae and Comamonadaceae. The abundance of hydrocarbon-degradation related genes increased by 10-1000 folds at the site where the spill accident occurred in multi-contaminated areas and was significantly related to 2-ring PAHs. Such changes of microbial community and hydrocarbon-degradation related genes together indicated the resilience of soil indigenous microbes toward multiple contamination events. Our results proved the significant change of bacterial community and huge shift of hydrocarbon-degradation related genes after the spill accident (multiple contamination events), and provided a deep insight into microbial response at industrial sites with a long period of contamination history.

Impacts of groundwater level fluctuation on soil microbial community, alkane degradation efficiency and alkane-degrading gene diversity in the critical zone: Evidence from an accelerated water table fluctuation simulation.

Petroleum hydrocarbons are hazardous to ecosystems and human health, commonly containing n-alkanes and polycyclic aromatic hydrocarbons. Previous researches have studied alkane degraders and degrading genes under aerobic or anaerobic conditions, but seldom discussed them in the intermittent saturation zone which is a connective area between the vadose zone and the groundwater aquifer with periodic alteration of oxygen and moisture. The present study investigated the difference in alkane degradation efficiency, bacterial community, and alkane degrading gene diversity in aerobic, anaerobic, and aerobic-anaerobic fluctuated treatments. All biotic treatments achieved over 90% of n-alkane removal after 120 days of incubation. The removal efficiencies of n-alkanes with a carbon chain length from 16 to 25 were much higher in anaerobic scenarios than those in aerobic scenarios, explained by different dominant microbes between aerobic and anaerobic conditions. The highest removal efficiency was found in fluctuation treatments, indicating an accelerated n-alkane biodegradation under aerobic-anaerobic alternation. In addition, the copy numbers of the 16S rRNA gene and two alkB genes (alkB-P and alkB-R) declined dramatically when switched from aerobic to anaerobic scenarios and oppositely from anaerobic to aerobic conditions. This suggested that water level fluctuation could notably change the presence of aerobic alkane degrading genes. Our results suggested that alkane degradation efficiency, soil microbial community, and alkane-degrading genes were all driven by water level fluctuation in the intermittent saturation zone, helping better understand the effects of seasonal water table fluctuation on the biodegradation of petroleum hydrocarbons in the subsurface environment.

In-vitro toxicity assessment of Eucalyptus robusta Smith extracts via whole-cell bioreporter.

Eucalyptus is widely planted in China for wood industries, and there are increasing concerns about its ecotoxicity in the environment. This study explored the in-vitro toxicity of Eucalyptus extracts by assessing the impacts of water-soluble and dimethylsulfoxide (DMSO)-soluble fractions via a whole-cell bioreporter, Acinetobacter baylyi ADPWH_recA. Compounds identified in Eucalyptus extracts included one tannin, two phenolic acids, four terpenoids, four glycosides, and five flavonoids. The leaf extracts contained more biological-active components than barks and roots. Genotoxicity induced by Eucalyptus extracts was mainly associated with water extracts (e.g., flavonoids, phenolic acids) instead of DMSO extracts. The significant cytotoxicity was explained by programmed cell death (PCD), suggested by the results of propidium iodide (PI) and 2',7'-dichlorofluorescein-diacetate (DCFH-DA) assays. Generally, water-soluble fractions contributed more toxicities than DMSO-soluble fractions, particularly at high concentrations. A robust linear regression was built between the compromised toxicity and PCD index (Compromised toxicity = -2.192 × PCD index + 2.219; R2 = 0.8886), suggesting a PCD-dependent compromised toxicity which was greatly underestimated. Our results implied non-neglectable ecotoxicological risks of Eucalyptus extracts, hinting at the possible magnified ecological impacts of its large-scale plantation and the potential adverse outcomes to the surrounding ecosystems.

Variation of bacterial community and alkane monooxygenase gene abundance in diesel n-alkane contaminated subsurface environment under seasonal water table fluctuation.

n-Alkanes, the main component of diesel fuel, are common light non-aqueous phase liquids (LNAPLs) that threaten ecological security. The subsurface from vadose zone, through fluctuating zone, to saturated zone, is a critical multi-interface earth layer which significantly affects the biodegradation processes of n-alkanes. A pilot-scale diesel contaminated aquifer column experiment has been undertaken to investigate the variations of bacterial community and alkane monooxygenase (alkB) gene abundance in these zones due to water-table fluctuations. The n-alkanes formed a layer immediately above the water table, and when this was raised, they were carried upwards through the fluctuating zone into the vadose zone. Water content and n-alkanes component C10-C12 are main factors influencing bacterial community variation in the vadose zone, while C10-C12 is a key driving factor shaping bacterial community in the fluctuating zone. The most abundant bacterial phyla at all three zones were Proteobacteria, Firmicutes and Actinobacteria, but moisture-niche selection determined their relative abundance. The intermittent wetting cycle resulted in higher abundance of Proteobacteria, and lower abundance of Actinobacteria in the vadose and fluctuating zones in comparison to the control column with a static water-table. The abundances of the alkB gene variants were relatively uniform in different zones, probably because the bacterial populations harboring alkB gene are habituated to biogenic n-alkanes rather than responding to diesel fuel contamination. The variation in the bacterial populations with height due to moisture-niche selection had very little effect on the alkB gene abundance, possibly because numerous species in both phyla (Proteobacteria and Actinobacteria) carry an alkB gene variant. Nevertheless, the drop in the water table caused a short-term spike in alkB gene abundance in the saturated zone, which is most likely associated with transport of solutes or colloids from the fluctuating zone to bacteria species in the saturated zone, so a fluctuating water table could potentially increase n-alkane biodegradation function.

Research on mental health intervention of college students based on music therapy / Pesquisa sobre intervenção na saúde mental de estudantes universitários com base na terapia musical / Investigación sobre la intervención en salud mental de estudiantes universitarios basada en música

ABSTRACT Although contemporary college students are in an era of stable environment and rich economy, the society has stricter requirements on their study and work. College students face various pressures from themselves and society, and psychological problems emerge one after another. From the practice abroad, music therapy itself has fully demonstrated the alleviation and improvement of people's mental health. Music is a part of human life, and its penetration and influence on human behavior is unparalleled by other cultural forces. Music does not only give people the enjoyment of beauty, but also influences people's mind, body and behavior through multiple psychological and physiological functions. The use of music therapy can better improve and optimize the ability of college students to eliminate bad emotions, so as to help students develop healthy a psychological state and establish a correct outlook on life and values. Based on this, this study explores the significance of contemporary college students' music therapy on mental health, and puts forward the orientation and implementation ways of college students' mental health education.

RESUMO Embora os universitários contemporâneos estejam em uma era de ambiente estável e economia rica, a sociedade tem exigências mais rigorosas em seu estudo e trabalho. Os estudantes universitários enfrentam várias pressões de si mesmos e da sociedade, e problemas psicológicos emergem um após o outro. Desde a prática no exterior, a própria terapia musical tem demonstrado plenamente o alívio e a melhoria da saúde mental das pessoas. A música faz parte do estilo de vida humano, e sua penetração e influência no comportamento humano é incomparável por outras forças culturais. A música não só dá às pessoas o prazer da beleza, mas também influencia a mente, o corpo e o comportamento das pessoas através de múltiplas funções psicológicas e fisiológicas. O uso da terapia musical pode melhorar e otimizar melhor a capacidade dos estudantes universitários de eliminar as emoções ruins, de modo a ajudá-los a desenvolver um estado psicológico saudável e estabelecer uma perspectiva correta sobre sua vida e seus valores. Com base nisso, este trabalho explora a relevância da terapia musical contemporânea entre estudantes universitários sobre sua saúde mental, e avança as formas de orientação e implementação da educação de saúde mental desses indivíduos.

RESUMEN Aunque los estudiantes universitarios contemporáneos se encuentran en una época de ambiente estable y economía rica, la sociedad tiene requisitos más estrictos respecto a sus estudios y trabajo. Los estudiantes universitarios enfrentan diversas presiones de sí mismos y de la sociedad, y los problemas psicológicos surgen uno tras otro. Desde la práctica en el extranjero, la musicoterapia en sí ha demostrado el alivio y la mejora de la salud mental de las personas. La música es parte del estilo de vida humano y su penetración e influencia en el comportamiento humano no tiene paralelo con otras fuerzas culturales. La música no solo brinda a las personas el disfrute de la belleza, sino que también influye en la mente, el cuerpo y el comportamiento de las personas a través de múltiples funciones psicológicas y fisiológicas. El uso de la musicoterapia puede mejorar y optimizar la capacidad de los estudiantes universitarios para eliminar las malas emociones, a fin de ayudarlos a desarrollar un estado psicológico saludable y establecer una perspectiva correcta de la vida y sus valores. Con base en esto, este trabajo de estudio explora la importancia de la musicoterapia en la salud mental de los estudiantes universitarios contemporáneos y presenta las formas de orientación e implementación de la educación en salud mental de dichos estudiantes.

Different acetonitrile degraders and degrading genes between anaerobic ammonium oxidation and sequencing batch reactor as revealed by stable isotope probing and magnetic-nanoparticle mediated isolation.

Microbial degraders play crucial roles in wastewater treatment processes, but their use is limited as most microbes are yet unculturable. Stable isotope probing (SIP) is a cultivation-independent technique identifying functional-yet-uncultivable microbes in ambient environment, but is unsatisfactory for substrates with low assimilation rate owing to the low isotope incorporation into DNA. In this study, we used acetonitrile as the target low-assimilation chemical in many wastewater treatment plants and attempted to identify the active acetonitrile degraders in the activated sludge, via DNA-SIP and magnetic-nanoparticle mediated isolation (MMI) which is another cultivation-independent approach without the requirement of substrate labeling. The two approaches identified different active acetonitrile degraders in a 3-day short-term anaerobic ammonium oxidation (ANAMMOX). MMI enriched significantly more acetonitrile-degraders than SIP, showing the advantages in identifying the active degraders for low-assimilation substrates. Sequencing batch reactor (SBR, 30-day degradation) helped in more incorporation of 15N-labeled acetonitrile into the active degraders, thus the same acetonitrile-degraders and acetonitrile-degrading genes were identified by SIP and MMI. Different acetonitrile degraders between ANAMMOX and SBR were attributed to the distinct hydrological conditions. Our study for the first time explored the succession of acetonitrile-degraders in wastewater and identified the active acetonitrile-degraders which could be further enriched for enhancing acetonitrile degradation performance. These findings provide new insights into the acetonitrile metabolic process in wastewater treatment plants and offer suggestive conclusions for selecting appropriate treatment strategy in wastewater management.

Separating and characterizing functional alkane degraders from crude-oil-contaminated sites via magnetic nanoparticle-mediated isolation.

Uncultivable microorganisms account for over 99% of all species on the planet, but their functions are yet not well characterized. Though many cultivable degraders for n-alkanes have been intensively investigated, the roles of functional n-alkane degraders remain hidden in the natural environment. This study introduces the novel magnetic nanoparticle-mediated isolation (MMI) technology in Nigerian soils and successfully separates functional microbes belonging to the families Oxalobacteraceae and Moraxellaceae, which are dominant and responsible for alkane metabolism in situ. The alkR-type n-alkane monooxygenase genes, instead of alkA- or alkP-type, were the key functional genes involved in the n-alkane degradation process. Further physiological investigation via a BIOLOG PM plate revealed some carbon (Tween 20, Tween 40 and Tween 80) and nitrogen (tyramine, l-glutamine and d-aspartic acid) sources promoting microbial respiration and n-alkane degradation. With further addition of promoter carbon or nitrogen sources, the separated functional alkane degraders significantly improved n-alkane biodegradation rates. This suggests that MMI is a promising technology for separating functional microbes from complex microbiota, with deeper insight into their ecological functions and influencing factors. The technique also broadens the application of the BIOLOG PM plate for physiological research on functional yet uncultivable microorganisms.

Application and reactivation of magnetic nanoparticles in Microcystis aeruginosa harvesting.

This study developed a magnetic nanoparticles (MNPs) harvesting and reactivation technique for rapid cyanobacteria Microcystis aeruginosa separation. The harvesting of raw MNPs achieved high efficiency of 99.6% with the MNPs dosage of 0.58g MNPs/g dry-biomass, but gradually decreased to 59.1% when directly reused 5 times. With extra ultrasonic chloroform:methanol solvent treatment, the MNPs can be effectively reactivated for M. aeruginosa harvesting with 60% efficiency after 5 times reactivation and the separation efficiency kept above 93% with 0.20g MNPs/g dry-biomass dosage. The cyanobacteria-MNPs complex can be effectively disrupted by ultrasonic chloroform:methanol solvent treatment and the zeta potential was recovered for MNPs electrostatic attraction. The MNPs adsorption followed the Langmuir isotherm, and the maximum adsorption capacity and Langmuir constant was 3.74g dry-biomass/g and 311.64L/g respectively. This MNPs reactivation technique can achieve low energy separation and reduce MNPs consumption by 67%, providing potential engineering implementation for cyanobacterial biomass harvesting.